NO ONE SPEND 4-5 HOURS FOR WESTERN BLOT ANYMORE
The western enhancer was designed to help you get your Western blot result quick and easily.
It only takes 1-1.5 hour to replace the 4-5 hours or overnight process of the classical western blotting.
Advantages:
1. The rapid process: the kit was designed to take one hour (1/2 hour for primary, 1/2 hour for secondary antibody binding and 8 min. washing to obtain a desired Western blot data.
2. The time saved: It takes only 1-1.5 hour instead of the 4-5 hours classic western process, with this kit, you can efficiently probe and re-probe membrane for Western, and Dot blots with multiple antibodies or with multiple concentration of an antibody for a desired result.
3. The money saving: One-Hour Western (OHW) is designed to increase the affinity of antibody to target antigen by 6-10 times in comparison to the classical Western method. And the used antibody in OHW antibody binding solution can be kept at 4˚C for 4-6 weeks.
Fig.1,Mouse tissue protein, used the actin 1: 30,000.
Fig.2-A, Mouse tissue protein, the antibody –β-Catenin,1ug/ml, 2 % Milk-HHWE, the result shows the high concentration antibody used.
Fig.2-B, The good result was obtained from the same membrane-Fig.2 A,re-probed with Anti-β-catenin (0.1ug/ml) in 2% milk-HHWE.
Fig.3,Human cell line protein, antibody-NFкB p50, E-10 (Santa Cruz, Cat#, sc-8414),0.66ug/ml in 2% Milk-HHWE.
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